{"status":"OK","data":{"advertisement":{"id":199806,"title":"Zapytanie ofertowe 2/SS/2023 z dnia 29.03.2023 r.","partial_offer_allowed":false,"publication_date":"2023-03-29","submission_deadline":"2023-05-04 23:59:59","planned_sign_date":null,"supplementary_orders":null,"terms_of_contract_change":"1. Wszelkie zmiany w umowie, kt\u00f3ra zostanie zawarta w wyniku post\u0119powania, wymagaj\u0105 formy pisemnej, pod rygorem niewa\u017cno\u015bci. \n2. Zamawiaj\u0105cy dopuszcza zmian\u0119 umowy w formie aneksu w sytuacji, kiedy nast\u0105pi ustawowa zmiana stawki VAT.","created_at":"2023-03-29 13:41:14","modified_at":"2023-05-05 00:00:03","contact_persons":[{"id":3621,"forename":"Ma\u0142gorzata","surname":"S\u0119ktas","phone_number":"+48 22 5936277","email":"m.sektas@cellis.eu","created_by":null,"created_at":"2026-03-20 13:48:06","modified_at":"2026-03-20 13:48:06","modified_by":null}],"orders":[{"id":330620,"estimated_value":null,"order_items":[{"id":375107,"description":"Produkcja i oczyszczanie rekombinowanego bia\u0142ka.\nProdukcja rekombinowanego bia\u0142ka  nieznakowanego (tag-free) w bakteryjnych systemach ekspresyjnych do cel\u00f3w bada\u0144 naukowych.\n\tProdukcja rekombinowanego bia\u0142ka obejmuje nast\u0119puj\u0105ce etapy:\n1.\tSynteza gen\u00f3w i optymalizacja kodonu w genie (Sekwencja bia\u0142ka zostanie dostarczona\nprzez Zamawiaj\u0105cego \u2013 d\u0142ugo\u015b\u0107 sekwencji 182 \u00b1 10 aminokwas\u00f3w),\n2.\tKlonowanie bezpo\u015brednie w wektorach plazmidowych,\n3.\tOcena ekspresji bia\u0142ek w ma\u0142ej skali w szczepie E.coli (minimalnie 2 \u2013 3 warunki ekspresji),\n4.\tWeryfikacja ekspresji bia\u0142ek za pomoc\u0105 elektroforezy w warunkach denaturuj\u0105cych \n(SDS-PAGE),\n5.\t10 l hodowla wysokiej g\u0119sto\u015bci kom\u00f3rek w systemie E.coli,\n6.\tKontrola jako\u015bci.\nWykonawca zobowi\u0105zuje si\u0119 przes\u0142a\u0107: \na)\tplazmid po klonowaniu,\nb)\traport z oceny ekspresji bia\u0142ek w ma\u0142ej skali,\nc)\traport ko\u0144cowy i certyfikat analizy zawieraj\u0105cy m.in.\n\t- specyfikacje czysto\u015bci,\n\t- badanie sterylno\u015bci,\n- poziom endotoksyn,\n- o\u015bwiadczenie o stabilno\u015bci.\nOczyszczanie bia\u0142ka z pasty bakteryjnej otrzymanej po hodowli wysokiej g\u0119sto\u015bci w systemie E.coli. Oczyszczanie bia\u0142ka obejmuje nast\u0119puj\u0105ce etapy:\n1. Oczyszczanie na du\u017c\u0105 skal\u0119:\na.\tLiza kom\u00f3rek w homogenizatorze,\nb.\tEtapowe oczyszczanie supernatantu poprzez stopniowe wytr\u0105canie w obecno\u015bci siarczanu amonu, dializ\u0119, ogrzewanie i ko\u0144cowe wytr\u0105canie w siarczanie amonu - kontrole SDS-PAGE,\nc.\tKo\u0144cowe oczyszczanie za pomoc\u0105 chromatografii SEC (size exclusion chromatography,) lub IEX (Ion Exchange Chromatography),\nd.\tWykonanie kontroli jako\u015bci produktu ko\u0144cowego za pomoc\u0105 m.in. spektroskopii UV-Vis \nlub Bradford assay oraz SDS-PAGE,\ne.\tWykonanie raportu z oczyszczenia na du\u017c\u0105 skal\u0119.\n\t\n\tWykonawca zobowi\u0105zuje si\u0119 dostarczy\u0107:\nI. Zat\u0119\u017cony oraz przefiltrowany 0.22 um produkt ko\u0144cowy w mo\u017cliwie najkr\u00f3tszym czasie \npo oczyszczeniu, utrzymuj\u0105c produkt w formie zamro\u017conej, w st\u0119\u017ceniu minimalnie 10 mg/ml \nw porcjach po 50 ml.\nII. Raport z oczyszczania na du\u017c\u0105 skal\u0119,\nIII. Dok\u0142adny, powtarzalny protok\u00f3\u0142 oczyszczania (metodologia).\n\nRecombinant protein production and purification.\nRecombinant protein production and purification.\nThe abovementioned order consist of:\nProduction of tag-free recombinant protein in bacterial expression systems for research purposes.\nRecombinant protein production involves the following steps:\n1.\tGene synthesis and codon optimization in a gene (Protein sequence will be provided\nby the Ordering Party - the length of the sequence 182 \u00b1 10 amino acids),\n2.\tDirect cloning in plasmid vectors,\n3.\tAssessment of small-scale protein expression in E.coli strain (a minimum of 2 - 3 expression conditions),\n4.\tVerification of protein expression by means of electrophoresis under denaturing conditions (SDS-PAGE),\n5.\t10 L high cell density culture in the E.coli system,\n6.\tQuality control.\nThe contractor undertakes to submit: \na)\tplasmid after cloning,\nb)\ta report on the evaluation of small-scale protein expression,\nc)\tfinal report and certificate of analysis containing, inter alia.\n- purity specifications,\n- sterility test,\n- endotoxin level,\n- a statement of stability.\nPurification of the protein from the bacterial paste obtained after high-density cultivation in the E.coli system. Protein purification involves the following steps:\n1. Large-scale purification:\na.\tCell disruption in a homogenizer,\nb.\tStepwise purification of supernatant by gradual precipitation in the presence of ammonium sulfate, dialysis, heating and final precipitation in ammonium sulfate \u2013 SDS-PAGE controls,\nc.\tFinal cleaning at size-exclusion chromatography or Ion Exchange Chromatography,\nd.\tPerforming a quality control of the final product using inter alia UV-Vis or Bradford assay spectroscopy and SDS-PAGE,\ne.\tPerforming of the purification at high scale report \nContractor declares to send:\nI.\tConcentrated and filtered 0.22 \u00b5m final product in the shortest possible time after purification, keeping the product frozen at a minimum concentration of 10 mg / ml \nin portions of 50 ml\nII.\tReport on high scale purification\nIII.\tMethodology - accurate reproducible purification protocol\n","warranty_period":null,"cpv_items":[{"id":8513,"code":"73100000-3","name":"Us\u0142ugi badawcze i eksperymentalno-rozwojowe"}],"timetable_items":[],"fulfillment_places":[{"country":"Polska","voivodeship":"mazowieckie","poviat":"Warszawa","commune":"\u017boliborz","locality":"Warszawa","created_by":null,"created_at":"2023-03-29 13:41:14","modified_at":"2023-03-29 13:41:14","modified_by":null,"id":422470,"type":{"id":1,"name":"adres"},"group_uuid":"c8b689a8-4c14-49bd-a58e-d86ce46bd74e"}],"participation_conditions":[{"id":1020610,"description":"Wykonawca musi posiada\u0107 udokumentowane do\u015bwiadczenie w realizacji us\u0142ug dotycz\u0105cych produkcji bia\u0142ek rekombinowanych w bakteryjnych systemach ekspresyjnych, potwierdzone przez udzia\u0142 w co najmniej pi\u0119ciu projektach/zam\u00f3wieniach w przeci\u0105gu ostatnich dw\u00f3ch lat.\nWykonawca musi posiada\u0107 niezb\u0119dn\u0105 wiedz\u0119 specjalistyczn\u0105 \u2013 co najmniej dwie osoby z minimum dwuletnim do\u015bwiadczeniem w produkcji bia\u0142ek rekombinowanych oraz oczyszczaniu pasty bakteryjnej ze stopniem \nco najmniej doktora oraz udokumentowane do\u015bwiadczenie w realizacji us\u0142ug dotycz\u0105cych oczyszczania pasty bakteryjnej, potwierdzone przez udzia\u0142 w co najmniej pi\u0119ciu projektach/zam\u00f3wieniach w przeci\u0105gu ostatnich dw\u00f3ch lat.\n\nContractor must have documented experience in the implementation of services for the production of recombinant protein in bacterial expression systems, confirmed by participation in at least five projects / orders within the last two years.\nContractor must have the necessary specialist knowledge - at least two people with at least two years of experience in the production of recombinant proteins and purification of bacterial paste with at least a PhD degree and documented experience in the implementation of bacterial paste purification services, confirmed by participation in at least five projects / orders within the last two years.","type":{"id":2,"name":"Wiedza i do\u015bwiadczenie"},"created_by":null,"created_at":"2023-03-29 13:41:14","modified_at":"2023-03-29 13:41:14","modified_by":null,"group_uuid":"563f6f15-bd44-47a7-b199-6d4ca04e73ed"},{"id":1020611,"description":"Wykonawca musi posiada\u0107 potencja\u0142 techniczny, zawieraj\u0105cy adekwatne techniczne zaplecze i niezb\u0119dny sprz\u0119t dedykowany do produkcji rekombinowanych bia\u0142ek w bakteryjnych systemach ekspresyjnych minimum spektrometr oraz aparat do elektroforezy bia\u0142ek w warunkach denaturuj\u0105cych (SDS/PAGE);\nWykonawca musi posiada\u0107 potencja\u0142 techniczny umo\u017cliwiaj\u0105cy oczyszczanie bia\u0142ka z pasty bakteryjnej, m.in.:\n- Homogenizator,\n- Fermentator,\n- Aparat do elektroforezy bia\u0142ek w warunkach denaturuj\u0105cych (SDS/PAGE),\n- Chromatograf - min. SEC lub IEX,\n- Nanospektrometr UV-VIS,\n- Wir\u00f3wka.\n\nContractor must have technical potential, including adequate technical facilities and the necessary equipment dedicated to the production of recombinant protein in bacterial expression systems minimum spectrometer and apparatus for protein electrophoresis in denaturing conditions (SDS / PAGE);\nContractor must have the technical potential to purify the protein from the bacterial paste, including i.e.:\n- Homogenizer \n- Fermenter\n- System for protein electrophoresis under denaturing conditions (SDS / PAGE)\n- Chromatograph - min.  SEC or IEX\n- UV-VIS spectrometer \n- Centrifuge","type":{"id":3,"name":"Potencja\u0142 techniczny"},"created_by":null,"created_at":"2023-03-29 13:41:14","modified_at":"2023-03-29 13:41:14","modified_by":null,"group_uuid":"fb855556-4e33-4359-86cd-353d43a4447f"},{"id":1020612,"description":"Wykonawca musi znajdowa\u0107 si\u0119 w sytuacji ekonomicznej i finansowej zapewniaj\u0105cej czasowe i zgodne z wymaganiami stawianymi w zapytaniu ofertowym wykonanie zam\u00f3wienia. \nO\u015bwiadczenie o zgodno\u015bci z warunkami udzia\u0142u w post\u0119powaniu znajduje si\u0119 w Za\u0142\u0105czniku 2 do Zapytania ofertowego 2/SS/2023.\n\nContractor must be in a business and financial situation that ensure timely and requirement compliant performance of the contract.\nThe statement about fulfilling conditions for participation in the proceedings is attached as Appendix 2 to this request for quotation 2/SS/2023.","type":{"id":5,"name":"Sytuacja ekonomiczna i finansowa"},"created_by":null,"created_at":"2023-03-29 13:41:14","modified_at":"2023-03-29 13:41:14","modified_by":null,"group_uuid":"763c186d-cd8b-4402-8cd8-e6cb3e0baa13"},{"id":1020613,"description":"1. Ka\u017cdy Oferent mo\u017ce z\u0142o\u017cy\u0107 jedn\u0105 ofert\u0119.\n2. Oferta musi by\u0107 z\u0142o\u017cona na Formularzu ofertowym (Za\u0142\u0105cznik 1 do Zapytania ofertowego 2/SS/2023). Oferta musi by\u0107 parafowana i podpisana przez nale\u017cycie umocowanego przedstawiciela Oferenta. \nW przypadku podpisania oferty przez osob\u0119 niewymienion\u0105 w dokumencie rejestrowym (ewidencyjnym) Oferenta, nale\u017cy do oferty do\u0142\u0105czy\u0107 stosowne pe\u0142nomocnictwo. \n3. Oferta musi zawiera\u0107 wszystkie wymagane przez Zamawiaj\u0105cego za\u0142\u0105czniki potwierdzone za zgodno\u015b\u0107 z orygina\u0142em przez nale\u017cycie umocowanego przedstawiciela Oferenta. \n4. Oferent zobowi\u0105zany jest z\u0142o\u017cy\u0107 wraz z ofert\u0105 aktualny odpis z krajowego rejestru s\u0105dowego lub z centralnej ewidencji i informacji o dzia\u0142alno\u015bci gospodarczej, wystawiony nie wcze\u015bniej ni\u017c trzy (3) miesi\u0105ce przed up\u0142ywem terminu sk\u0142adania ofert, w celu wykazania braku podstaw do wykluczenia. \n\n1. Each Bidder may submit only one offer.\n2. Offer should be prepared in the bid form (Appendix 1 to the request for quotation 2/SS/2023). The offer must be initialled and signed by the authorised representative of the Bidder.\nIf the offer would be signed by the person not listed in registered documents of the Contractor, the offer shall be accompanied by the appropriate power of attorney.\n3. Offer must include all attachments required by the Ordering Party confirmed to be true by the authorised representative of the Bidder.  \n4. Contractor must submit with the offer copy (scan) of relevant Register of Business Activity, applicable to country of residence of Contractor, issued not earlier than three (3) months before the deadline for submission the offer to demonstrate the absence of grounds for exclusion.","type":{"id":6,"name":"Lista wymaganych dokument\u00f3w/o\u015bwiadcze\u0144"},"created_by":null,"created_at":"2023-03-29 13:41:14","modified_at":"2023-03-29 13:41:14","modified_by":null,"group_uuid":"2d4eff26-f1e1-4668-a64a-d5f179b12525"},{"id":1020614,"description":"Wykonawca nie mo\u017ce by\u0107 powi\u0105zany osobowo lub kapita\u0142owo z Zamawiaj\u0105cym.\nPrzez powi\u0105zania kapita\u0142owe lub osobowe rozumie si\u0119 wzajemne powi\u0105zania mi\u0119dzy beneficjentem lub osobami upowa\u017cnionymi do zaci\u0105gania zobowi\u0105za\u0144 w imieniu beneficjenta lub osobami wykonuj\u0105cymi w imieniu beneficjenta czynno\u015bci zwi\u0105zane z przeprowadzeniem procedury wyboru wykonawcy \na wykonawc\u0105, polegaj\u0105ce w szczeg\u00f3lno\u015bci na:\na)\tuczestniczeniu w sp\u00f3\u0142ce jako wsp\u00f3lnik sp\u00f3\u0142ki cywilnej lub sp\u00f3\u0142ki osobowej,\nb)\tposiadaniu co najmniej 10% udzia\u0142\u00f3w lub akcji, o ile ni\u017cszy pr\u00f3g nie wynika z przepis\u00f3w prawa lub nie zosta\u0142 okre\u015blony przez IZ PO,\nc)\tpe\u0142nieniu funkcji cz\u0142onka organu nadzorczego lub zarz\u0105dzaj\u0105cego, prokurenta, pe\u0142nomocnika,\nd)\tpozostawaniu w zwi\u0105zku ma\u0142\u017ce\u0144skim, w stosunku pokrewie\u0144stwa lub powinowactwa w linii prostej, pokrewie\u0144stwa drugiego stopnia lub powinowactwa drugiego stopnia w linii bocznej lub w stosunku przysposobienia, opieki lub kurateli.\n\nOferent zobowi\u0105zany jest z\u0142o\u017cy\u0107 stosowne o\u015bwiadczenie zawarte w Za\u0142\u0105czniku 3 do Zapytania ofertowego nr 2/SS/2023.\nContractor may not be personally or equity related to the Ordering Party.\nEquity or personal relationship is understood as relations between the Ordering Party or individuals authorized to take commitments on behalf of the Ordering Party or those acting on behalf of the Ordering Party in order to prepare and implement the contractor selection procedure, and the Contractor, including in particular:\na)\tBeing a partner to civil law partnership or commercial law partnership,\nb)\tHolding at least 10% of shares, if lower threshold does not result from law or is not specified by Managing Authority for the Operational Programme,\nc)\tBeing a member of a supervisory or management corporate body, a holder of general commercial power of attorney, an authorised representative,\nd)\tBeing married to, being in direct consanguinity or affinity, second degree consanguinity or affinity \nof the second degree collaterally or by adoption or guardianship. \n\nThe Bidder is obliged to submit a relevant statement contained in Annex 3 to the request for quotation 2/SS/2023.","type":{"id":7,"name":"Dodatkowe warunki udzia\u0142u"},"created_by":null,"created_at":"2023-03-29 13:41:14","modified_at":"2023-03-29 13:41:14","modified_by":null,"group_uuid":"5b0ca4f8-89f1-4988-b862-6f8e63d8bbc6"}],"category":{"id":1,"name":"Us\u0142uga"},"subcategory":{"id":4,"name":"Us\u0142ugi badawcze"},"created_by":null,"created_at":"2023-03-29 13:41:14","modified_at":"2023-03-29 13:41:14","modified_by":null,"group_uuid":"a9ef0688-b2c9-4e22-8c07-098e533d5b0e"}],"evaluation_criteria":[{"id":574361,"price_criterion":true,"description":"\u2022\tCena \u2013 80 %\nKryterium \u201eCena\u201d oceniane b\u0119dzie nast\u0119puj\u0105co: \nPi(C) \u2013 liczba punkt\u00f3w jakie otrzyma oferta \u201ei\u201d za kryterium \u201eCena\u201d\nCmin \u2013 najni\u017csza cena spo\u015br\u00f3d wszystkich wa\u017cnych i nieodrzuconych ofert\nCi \u2013 cena oferty \u201ei\u201d \nPi(C) = (Cmin : Ci) x 80\nW kryterium Cena Oferent mo\u017ce uzyska\u0107 80 pkt.","created_by":null,"created_at":"2023-03-29 13:41:14","modified_at":"2023-03-29 13:41:14","modified_by":null,"group_uuid":"1192683a-224c-4010-8871-99c4b70aae68"},{"id":574362,"price_criterion":false,"description":"\u2022\tTermin realizacji zam\u00f3wienia \u2013 20%\nKryterium \u201eTermin realizacji zam\u00f3wienia\u201d oceniane b\u0119dzie w nast\u0119puj\u0105cy spos\u00f3b: \n1 \u2013 3 tygodnie \u2013 20 punkt\u00f3w\n4 \u2013 6 tygodni \u2013 10 punkt\u00f3w\npowy\u017cej 6  tygodni \u2013 0 punkt\u00f3w","created_by":null,"created_at":"2023-03-29 13:41:14","modified_at":"2023-03-29 13:41:14","modified_by":null,"group_uuid":"508344b1-8d5a-428c-a5b1-1a35b4bdfbcc"}],"is_variant":false,"title":"Produkcja i oczyszczanie rekombinowanego bia\u0142ka","order_node":{"id":251708},"created_by":null,"created_at":"2023-03-29 13:41:14","modified_at":"2023-03-29 13:41:14","modified_by":null}],"created_by":{"id":4346,"contact_persons":[]},"modified_by":{"modified_by":null,"id":1,"contact_persons":[]},"status":{"id":2,"label":"PUBLISHED","name":"Opublikowane"},"advertiser_address_details":{"name":"Cellis Sp. z o.o.","identification_number_type":"nip","identification_number":"5252640606","address":{"country":"Polska","voivodeship":"mazowieckie","poviat":"Warszawa","commune":"\u017boliborz","locality":"Warszawa","created_by":null,"created_at":null,"modified_at":null,"modified_by":null,"id":519848,"street":"Genera\u0142a Zaj\u0105czka","number_description":"28","postcode":"01-510"},"created_by":{"id":4346,"contact_persons":[]},"created_at":"2023-03-29 13:41:14","modified_at":"2023-03-29 13:41:14","modified_by":{"id":4346,"contact_persons":[]},"id":200264},"advertisement":{"id":152546,"number":"2023-4346-152546","publication_date":"2023-03-29","created_by":{"id":4346,"contact_persons":[]},"created_at":"2023-03-29 13:41:14","modified_at":"2023-05-05 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